Hotspot

trnH (GUG)-psbA

LSC · 214-603 · 390 bp

Back to Eleutherococcus

Pi 0.0154
MarkerSeek score 47.30
Primer available yes
Label trnH (GUG)-psbA

Feature Metadata

label_name
trnH (GUG)-psbA
feature_type
igs
region
LSC
length_bp
390
variable_sites
17
pi
0.0154
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH (GUG)-psbA_p1 GATCCACTTGGCTACATCCG CCTCTAGACCTAGCTGCTGT 55.0 55.0 58.1 57.9 455-466 bp 1.0 83.5
trnH (GUG)-psbA_p2 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 458-469 bp 1.0 83.4
trnH (GUG)-psbA_p3 CAATCCACTGCCTTGATCCA CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 469-480 bp 1.0 83.3
trnH (GUG)-psbA_p4 AATCCACTGCCTTGATCCAC CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 468-479 bp 1.0 83.3
trnH (GUG)-psbA_p5 ACAATCCACTGCCTTGATCC CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 470-481 bp 1.0 83.3