Hotspot

trnG-GCC

LSC · 12465-12535 · 71 bp

Back to Eleusine

Pi 0.0141

MarkerSeek score 60.60

Primer available yes

Label trnG-GCC

Feature Metadata

label_name: trnG-GCC
feature_type: tRNA
region: LSC
length_bp: 71
variable_sites: 1
pi: 0.0141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC_p1	AAAATGAAACGGTCGACCCA	AGGAAGGGATACGGTACTAGAC	45.0	50.0	58.0	57.9	264 bp	1.0	63.3
trnG-GCC_p2	AAAATGAAACGGTCGACCCA	GGAAGGGATACGGTACTAGACT	45.0	50.0	58.0	57.9	263 bp	1.0	63.3
trnG-GCC_p3	GACCCAGGCGGATATATCCT	AGGAAGGGATACGGTACTAGAC	55.0	50.0	58.1	57.9	231 bp	1.0	62.5
trnG-GCC_p4	GACCCAGGCGGATATATCCT	GGAAGGGATACGGTACTAGACT	55.0	50.0	58.1	57.9	230 bp	1.0	62.5
trnG-GCC_p5	CCGTAGCGAGCATAGTTCAA	GGAAGGGATACGGTACTAGACT	50.0	50.0	57.8	57.9	198 bp	1.0	62.0