Hotspot

rps4

LSC · 45557-46162 · 606 bp

Back to Eleusine

Pi 0.00165

MarkerSeek score 59.70

Primer available yes

Label rps4

Feature Metadata

label_name: rps4
feature_type: gene
region: LSC
length_bp: 606
variable_sites: 1
pi: 0.00165
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4_p1	AGGTCGATTTAGTTCGGGGA	ACAATGCAATGAAATTTATTGTGT	50.0	25.0	58.1	54.8	731 bp	1.0	40.1
rps4_p2	TTAGTTCGGGGAAAGGGGAA	ACAATGCAATGAAATTTATTGTGT	50.0	25.0	58.3	54.8	723 bp	1.0	40.1
rps4_p3	TTTAGTTCGGGGAAAGGGGA	ACAATGCAATGAAATTTATTGTGT	50.0	25.0	58.3	54.8	724 bp	1.0	40.1
rps4_p4	GAAAGGGGAAGGGGGAAAAA	ACAATGCAATGAAATTTATTGTGT	50.0	25.0	57.6	54.8	713 bp	1.0	40.1
rps4_p5	GGTCGATTTAGTTCGGGGAA	ACAATGCAATGAAATTTATTGTGT	50.0	25.0	57.3	54.8	730 bp	1.0	40.1