Hotspot

petA-psbJ

LSC · 60904-61677 · 774 bp

Back to Eleusine

Pi 0.00129

MarkerSeek score 59.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 774
variable_sites: 1
pi: 0.00129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTCCAAGGCCTTTTGTTCT	ACGGTTCGTATTCTGGATTGG	50.0	47.6	58.7	58.1	918 bp	1.0	65.8
petA-psbJ_p2	GATCCATTACGCGTCCAAGG	ACGGTTCGTATTCTGGATTGG	55.0	47.6	58.8	58.1	929 bp	1.0	65.0
petA-psbJ_p3	CGTCCAAGGCCTTTTGTTCT	GGTTCGTATTCTGGATTGGGT	50.0	47.6	58.7	57.7	916 bp	1.0	64.4
petA-psbJ_p4	CGTCCAAGGCCTTTTGTTCT	GCTGATAGGTACTGTAACCGG	50.0	52.4	58.7	57.6	974 bp	1.0	63.7
petA-psbJ_p5	CGTCCAAGGCCTTTTGTTCT	GGCTGATAGGTACTGTAACCG	50.0	52.4	58.7	57.6	975 bp	1.0	63.7