Hotspot

trnG-UCC-trnT-GGU

LSC · 13839-14793 · 955 bp

Back to Eleusine

Pi 0.00105
MarkerSeek score 59.40
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
955
variable_sites
1
pi
0.00105
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 ACGAATCACACTTTTACCACT CTTTCCGTGGCATTACTCCA 38.1 50.0 55.5 57.9 1094 bp 1.0 50.9
trnG-UCC-trnT-GGU_p2 ACGAATCACACTTTTACCACT GGTCTTTCCGTGGCATTACT 38.1 50.0 55.5 57.9 1097 bp 1.0 50.9
trnG-UCC-trnT-GGU_p3 AGAACGAATCACACTTTTACCAC CTTTCCGTGGCATTACTCCA 39.1 50.0 57.3 57.9 1097 bp 1.0 49.9
trnG-UCC-trnT-GGU_p4 GAACGAATCACACTTTTACCACT CTTTCCGTGGCATTACTCCA 39.1 50.0 57.3 57.9 1096 bp 1.0 49.9
trnG-UCC-trnT-GGU_p5 GAACGAATCACACTTTTACCACT GGTCTTTCCGTGGCATTACT 39.1 50.0 57.3 57.9 1099 bp 1.0 49.8