Hotspot

psbK-psbI

LSC · 7464-7854 · 391 bp

Back to Eleusine

Pi 0.00512

MarkerSeek score 57.30

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 391
variable_sites: 2
pi: 0.00512
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CGTGGATGTTATGCCTGTCA	TTTACTCCTCACGTCCAGGA	50.0	50.0	58.0	57.7	582 bp	1.0	77.7
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TTTACTCCTCACGTCCAGGA	50.0	50.0	57.9	57.7	533 bp	1.0	77.5
psbK-psbI_p3	ACCCGAAGCTTATGCCATTT	TTTACTCCTCACGTCCAGGA	45.0	50.0	57.9	57.7	612 bp	1.0	76.7
psbK-psbI_p4	TTAGCGTTTGTTTGGCAAGC	TTTACTCCTCACGTCCAGGA	45.0	50.0	58.2	57.7	542 bp	1.0	76.7
psbK-psbI_p5	CGTGGATGTTATGCCTGTCA	ACTCCTCACGTCCAGGATTA	50.0	50.0	58.0	57.5	579 bp	1.0	75.9