Hotspot

rps16-trnQ-UUG

LSC · 5570-6864 · 1295 bp

Back to Eleusine

Pi 0.00154

MarkerSeek score 59.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1295
variable_sites: 2
pi: 0.00154
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1368 bp	1.0	78.4
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	CGCATAAAAGAAATGGGGCG	45.0	50.0	58.1	58.2	1429 bp	1.0	77.7
rps16-trnQ-UUG_p3	TCATCTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	1379 bp	1.0	76.5
rps16-trnQ-UUG_p4	TCATCTCCTTCAAGTCGCAC	CGCATAAAAGAAATGGGGCG	50.0	50.0	57.6	58.2	1440 bp	1.0	75.9
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1372 bp	1.0	73.7