Hotspot

matK

LSC · 2092-3612 · 1521 bp

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Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1521
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TTCCAAGGTTTCCTAACAAA	ACCCCCAAAATCCTCTACCT	35.0	50.0	52.5	57.6	1582 bp	1.0	40.0
matK_p2	TTTCCAAGGTTTCCTAACAA	ACCCCCAAAATCCTCTACCT	35.0	50.0	52.5	57.6	1583 bp	1.0	40.0
matK_p3	TTTCCAAGGTTTCCTAACAAA	ACCCCCAAAATCCTCTACCT	33.3	50.0	53.4	57.6	1583 bp	1.0	40.0
matK_p4	ATTTCCAAGGTTTCCTAACA	ACCCCCAAAATCCTCTACCT	35.0	50.0	52.2	57.6	1584 bp	1.0	40.0
matK_p5	ATTTCCAAGGTTTCCTAACAA	ACCCCCAAAATCCTCTACCT	33.3	50.0	53.2	57.6	1584 bp	1.0	40.0