Hotspot

petA-psbJ

LSC · 62383-63179 · 797 bp

Back to Elephantopus

Pi 0.00125

MarkerSeek score 59.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 797
variable_sites: 1
pi: 0.00125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTACAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	58.0	57.6	935 bp	1.0	76.8
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.9	57.6	925 bp	1.0	76.8
petA-psbJ_p3	TCCATTACGTGTACAAGGCC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.6	57.6	941 bp	1.0	74.0
petA-psbJ_p4	CCATTACGTGTACAAGGCCT	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.6	57.6	940 bp	1.0	74.0
petA-psbJ_p5	GCCTTTTGTTCTTCTTCGCA	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.2	57.6	924 bp	1.0	71.0