Hotspot

psbM-trnD-GUC

LSC · 10834-11487 · 654 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
654
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGAATGAACAGTGCAGTAGC TTCAATAGGCAAGAGCACCG 45.0 50.0 55.8 58.3 765 bp 1.0 60.1
psbM-trnD-GUC_p2 AGAATGAACAGTGCAGTAGC TCCCCGGGATTGTAGTTCAA 45.0 50.0 55.8 58.3 780 bp 1.0 59.4
psbM-trnD-GUC_p3 AGAATGAACAGTGCAGTAGC TTCCCCGGGATTGTAGTTCA 45.0 50.0 55.8 58.3 781 bp 1.0 59.4
psbM-trnD-GUC_p4 AGGAACTAGAATGAACAGTGCA TTCAATAGGCAAGAGCACCG 40.9 50.0 57.4 58.3 772 bp 1.0 56.9
psbM-trnD-GUC_p5 AGGAACTAGAATGAACAGTGCA TCCCCGGGATTGTAGTTCAA 40.9 50.0 57.4 58.3 787 bp 1.0 56.3