Hotspot

ycf1

SSC · 125640-131108 · 5469 bp

Back to Elatostematoides

Pi 0.00659

MarkerSeek score 56.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5469
variable_sites: 36
pi: 0.00659
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGGGAATGTGCATTTGCTTC	TCCCGCGGACAAATTTGTAA	50.0	45.0	58.0	58.1	686 bp	1.0	80.2
ycf1_p2	AAAAGTGGGGGAATGTGCAT	TCCCGCGGACAAATTTGTAA	45.0	45.0	58.0	58.1	693 bp	1.0	80.1
ycf1_p3	AAAGTGGGGGAATGTGCATT	TCCCGCGGACAAATTTGTAA	45.0	45.0	58.0	58.1	692 bp	1.0	80.1
ycf1_p4	AAGTGGGGGAATGTGCATTT	TCCCGCGGACAAATTTGTAA	45.0	45.0	58.0	58.1	691 bp	1.0	80.1
ycf1_p5	TGCCAAGGTTTCAGACGAAA	TCCCGCGGACAAATTTGTAA	45.0	45.0	58.0	58.1	1190 bp	1.0	79.7