Hotspot

petG

LSC · 67472-67585 · 114 bp

Back to Elatostematoides

Pi 0

MarkerSeek score 21.30

Primer available yes

Label petG

Feature Metadata

label_name: petG
feature_type: gene
region: LSC
length_bp: 114
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 21.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG_p1	ATTTTTGTTAGGTCCGCCGT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.1	58.0	677-682 bp	1.0	80.6
petG_p2	ATTTTTGTTAGGTCCGCCGT	AGAACGTGGGTCTCCAAAAC	45.0	50.0	58.1	58.0	655-660 bp	1.0	80.5
petG_p3	ATTTTTGTTAGGTCCGCCGT	CAAGACAGGTTGGGTCACAA	45.0	50.0	58.1	57.9	717-722 bp	1.0	80.2
petG_p4	ATTTTTGTTAGGTCCGCCGT	ATGTCCCGTTCTGTCTGAGA	45.0	50.0	58.1	58.1	1116-1120 bp	1.0	79.6
petG_p5	ATTTTTGTTAGGTCCGCCGT	TTTGACCTCCTGCTTGCTTT	45.0	45.0	58.1	57.9	551 bp	0.5	58.6