Hotspot

ndhE

SSC · 119554-119859 · 306 bp

Back to Elatostematoides

Pi 0

MarkerSeek score 22.60

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 22.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	TGTCTGGTGAATACTACTTATCA	CATCAATTGAACGCGTTGCT	34.8	45.0	54.3	58.0	406 bp	1.0	40.0
ndhE_p2	TGTCTGGTGAATACTACTTATCA	TCATCAATTGAACGCGTTGC	34.8	45.0	54.3	58.0	407 bp	1.0	40.0
ndhE_p3	TGTCTGGTGAATACTACTTATCA	AATTGAACGCGTTGCTTTGT	34.8	40.0	54.3	57.8	402 bp	1.0	40.0
ndhE_p4	TGTCTGGTGAATACTACTTATCA	ATTGAACGCGTTGCTTTGTT	34.8	40.0	54.3	57.8	401 bp	1.0	40.0
ndhE_p5	TTTGTCTGGTGAATACTACTT	CATCAATTGAACGCGTTGCT	33.3	45.0	52.1	58.0	408 bp	1.0	40.0