Hotspot

ccsA-ndhD

SSC · 117139-117407 · 269 bp

Back to Elatostematoides

Pi 0.0373

MarkerSeek score 66.00

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 269
variable_sites: 10
pi: 0.0373
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGGATATGCTACTTTGGGGT	TCAGTTGAGAAAGTCGAAGCT	45.0	42.9	55.9	57.3	394-395 bp	1.0	51.4
ccsA-ndhD_p2	TGGATATGCTACTTTGGGGTT	TCAGTTGAGAAAGTCGAAGCT	42.9	42.9	56.7	57.3	394-395 bp	1.0	49.7
ccsA-ndhD_p3	TTGGATATGCTACTTTGGGGT	TCAGTTGAGAAAGTCGAAGCT	42.9	42.9	56.7	57.3	395-396 bp	1.0	49.7
ccsA-ndhD_p4	TGGATATGCTACTTTGGGGT	AGTTGAGAAAGTCGAAGCTCT	45.0	42.9	55.9	57.0	392-393 bp	1.0	49.1
ccsA-ndhD_p5	TGGATATGCTACTTTGGGGT	CAGTTGAGAAAGTCGAAGCTC	45.0	47.6	55.9	56.9	393-394 bp	1.0	48.8