Hotspot

ycf1-ndhF

SSC · 111535-112138 · 604 bp

Back to Elatostematoides

Pi 0.00331
MarkerSeek score 48.20
Primer available yes
Label ycf1-ndhF

Feature Metadata

label_name
ycf1-ndhF
feature_type
igs
region
SSC
length_bp
604
variable_sites
2
pi
0.00331
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-ndhF_p1 ACGAAGGGGACTAAACAGGA GGTATGGGTTCGTATCGAGC 50.0 55.0 58.0 57.9 1507-1509 bp 1.0 79.9
ycf1-ndhF_p2 GCAGAATACCGTCACCCATT GGTATGGGTTCGTATCGAGC 50.0 55.0 58.0 57.9 1608-1610 bp 1.0 79.7
ycf1-ndhF_p3 ACAGGAACAAGAGGGATCCA GGTATGGGTTCGTATCGAGC 50.0 55.0 58.0 57.9 1493-1495 bp 1.0 79.7
ycf1-ndhF_p4 AAGAGGGATCCACCGAAGAA GGTATGGGTTCGTATCGAGC 50.0 55.0 58.0 57.9 1485-1487 bp 1.0 79.7
ycf1-ndhF_p5 ACGAAGGGGACTAAACAGGA ACTTTTCGGGGCCACTTTAG 50.0 50.0 58.0 58.1 1604-1606 bp 1.0 79.6