Hotspot

petG-trnW-CCA

LSC · 67586-67696 · 111 bp

Back to Elatostematoides

Pi 0.108

MarkerSeek score 87.50

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 111
variable_sites: 12
pi: 0.108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 87.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	ACAATACAGACGTGGCGATC	AGAACGTGGGTCTCCAAAAC	50.0	50.0	58.1	58.0	198-203 bp	1.0	83.9
petG-trnW-CCA_p2	CAATACAGACGTGGCGATCA	AGAACGTGGGTCTCCAAAAC	50.0	50.0	58.1	58.0	197-202 bp	1.0	83.9
petG-trnW-CCA_p3	ACAATACAGACGTGGCGATC	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.1	58.0	220-225 bp	1.0	83.6
petG-trnW-CCA_p4	CAATACAGACGTGGCGATCA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.1	58.0	219-224 bp	1.0	83.6
petG-trnW-CCA_p5	ACAATACAGACGTGGCGATC	CAAGACAGGTTGGGTCACAA	50.0	50.0	58.1	57.9	260-265 bp	1.0	82.6