Hotspot

psbE-petL

LSC · 66065-67274 · 1210 bp

Back to Elatostematoides

Pi 0.00579

MarkerSeek score 62.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1210
variable_sites: 7
pi: 0.00579
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	ACCGATAAATAGAGCTGCGG	45.0	50.0	58.0	57.6	1397-1401 bp	1.0	76.7
psbE-petL_p2	TGACAAATAACCAACCCGCA	CCGATAAATAGAGCTGCGGT	45.0	50.0	58.0	57.6	1396-1400 bp	1.0	76.7
psbE-petL_p3	CAACCCGCAATGAATAGGGA	ACCGATAAATAGAGCTGCGG	50.0	50.0	57.9	57.6	1386-1390 bp	1.0	76.5
psbE-petL_p4	CAACCCGCAATGAATAGGGA	CCGATAAATAGAGCTGCGGT	50.0	50.0	57.9	57.6	1385-1389 bp	1.0	76.5
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	CCGATAAATAGAGCTGCGGT	50.0	50.0	57.8	57.6	1308-1312 bp	1.0	75.7