Hotspot

ndhC-trnV-UAC

LSC · 51507-52076 · 570 bp

Back to Elatostematoides

Pi 0.0105

MarkerSeek score 64.20

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 570
variable_sites: 6
pi: 0.0105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	701 bp	1.0	78.0
ndhC-trnV-UAC_p2	CTGGTCCTTTGCTAATCGGG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.3	57.8	718 bp	1.0	76.4
ndhC-trnV-UAC_p3	CCTTTGCTAATCGGGGCTAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	713 bp	1.0	76.4
ndhC-trnV-UAC_p4	GGGGCTAAAACTCCGGAAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	700 bp	1.0	75.2
ndhC-trnV-UAC_p5	TCCTTTGCTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.5	57.8	714 bp	1.0	75.1