Hotspot

ndhK-ndhC

LSC · 51102-51143 · 42 bp

Back to Elatostematoides

Pi 0.0238

MarkerSeek score 66.20

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 42
variable_sites: 1
pi: 0.0238
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	AGAGAAGCGGCCATAAACTG	CATGGCGAAAAGGAGCATTG	50.0	50.0	58.0	58.1	192 bp	1.0	79.5
ndhK-ndhC_p2	AGAGAAGCGGCCATAAACTG	TGGGCAATGAGTTTCGATGT	50.0	45.0	58.0	57.8	280 bp	1.0	78.5
ndhK-ndhC_p3	AGAGAAGCGGCCATAAACTG	CGATGTATTGGGGGTATCCG	50.0	55.0	58.0	57.6	266 bp	1.0	77.0
ndhK-ndhC_p4	AGAGAAGCGGCCATAAACTG	GCGAAAAGGAGCATTGGAAT	50.0	45.0	58.0	57.1	188 bp	1.0	72.6
ndhK-ndhC_p5	AGAGAAGCGGCCATAAACTG	TATGCATGGCGAAAAGGAGC	50.0	50.0	58.0	59.0	196 bp	1.0	72.4