Hotspot

petN-psbM

LSC · 29420-30159 · 740 bp

Back to Elatostematoides

Pi 0.0149

MarkerSeek score 67.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 740
variable_sites: 11
pi: 0.0149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCACTGTTCATTCTAGTCCC	50.0	47.6	58.2	57.7	896 bp	1.0	69.1
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	902 bp	1.0	68.5
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	TGCACTGTTCATTCTAGTCCC	50.0	47.6	57.6	57.7	835 bp	1.0	67.6
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	841 bp	1.0	67.0
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	GCACTGTTCATTCTAGTCCCT	50.0	47.6	58.2	57.4	895 bp	1.0	66.8