Hotspot

trnC-GCA-petN

LSC · 28405-29329 · 925 bp

Back to Elatostematoides

Pi 0.00113

MarkerSeek score 63.30

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 925
variable_sites: 1
pi: 0.00113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTTGTATCGTTTTGGCGGC	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.9	58.2	1011-1046 bp	1.0	81.3
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	955-990 bp	1.0	81.3
trnC-GCA-petN_p3	GGGTGTCGCCTGATAAACAA	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.2	58.2	937-972 bp	1.0	80.5
trnC-GCA-petN_p4	TTTTGTATCGTTTTGGCGGC	AGAGTCCACTTCTTCCCCAT	45.0	50.0	57.9	57.6	1072-1107 bp	1.0	79.6
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	1016-1051 bp	1.0	79.5