Hotspot

rps16-trnQ-UUG

LSC · 6390-7125 · 736 bp

Back to Elatostematoides

Pi 0.0014

MarkerSeek score 62.40

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 736
variable_sites: 1
pi: 0.0014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	796-809 bp	1.0	80.6
rps16-trnQ-UUG_p2	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	811-824 bp	1.0	78.7
rps16-trnQ-UUG_p3	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	810-823 bp	1.0	78.7
rps16-trnQ-UUG_p4	TGGGTGTAAGAATCCACAGC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.5	58.1	830-843 bp	1.0	77.7
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	GGGTTTTGGTCCCGGTATTC	45.0	55.0	58.1	58.5	817-830 bp	1.0	76.9