Hotspot

ccsA-ndhD

SSC · 115741-116013 · 273 bp

Back to Elatostema

Pi 0.1

MarkerSeek score 75.40

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 273
variable_sites: 105
pi: 0.1
unique_haplotype_count: 38
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ATGTAATAACCGCGCCAAGT	GCGCGAATTGTTTCTTTCGA	45.0	45.0	58.0	58.1	1071-1109 bp	0.837209	83.5
ccsA-ndhD_p2	GCTGGGCGAGATTCAATACA	GCGCGAATTGTTTCTTTCGA	50.0	45.0	58.0	58.1	815-853 bp	0.813953	83.3
ccsA-ndhD_p3	GCTGGGCGAGATTCAATACA	TCAACGGGGCCATTTTACAA	50.0	45.0	58.0	58.0	1209-1247 bp	0.395349	64.5
ccsA-ndhD_p4	ATGTAATAACCGCGCCAAGT	TCAACGGGGCCATTTTACAA	45.0	45.0	58.0	58.0	1465-1503 bp	0.372093	63.3
ccsA-ndhD_p5	CTGCAATTGTGGCTTCGATG	TCAACGGGGCCATTTTACAA	50.0	45.0	58.1	58.0	907-908 bp	0.325581	55.9