Hotspot

ndhF-rpl32

SSC · 112605-113478 · 874 bp

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Pi 0.0712

MarkerSeek score 77.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 874
variable_sites: 256
pi: 0.0712
unique_haplotype_count: 42
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CCGGGTGAACCAAAAACCTA	CGCTGCCCAATATCCTTTCT	50.0	50.0	58.0	58.0	1107-1490 bp	1.0	91.7
ndhF-rpl32_p2	TGTTGCGGTGGGAAAAAGTA	CGCTGCCCAATATCCTTTCT	45.0	50.0	57.9	58.0	702-1085 bp	1.0	91.3
ndhF-rpl32_p3	CCGGGTGAACCAAAAACCTA	AACGCTGCCCAATATCCTTT	50.0	45.0	58.0	57.9	1109-1492 bp	1.0	90.7
ndhF-rpl32_p4	CTCCAACGCGATTAGTCACA	CGCTGCCCAATATCCTTTCT	50.0	50.0	58.0	58.0	1159-1522 bp	0.837209	85.2
ndhF-rpl32_p5	CTCCAACGCGATTAGTCACA	AACGCTGCCCAATATCCTTT	50.0	45.0	58.0	57.9	1161-1524 bp	0.837209	84.3