Hotspot

accD-psaI

LSC · 59084-59774 · 691 bp

Back to Elatostema

Pi 0.0409

MarkerSeek score 78.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 691
variable_sites: 177
pi: 0.0409
unique_haplotype_count: 40
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TGAACCCGATGCCTACATTG	TCGAGACCCCGCTATAAGTT	50.0	50.0	58.0	57.9	1486-1592 bp	1.0	91.2
accD-psaI_p2	TGAACCCGATGCCTACATTG	TTCGAGACCCCGCTATAAGT	50.0	50.0	58.0	57.9	1487-1593 bp	1.0	91.2
accD-psaI_p3	CTACTACAGGTGGGGTGACA	TCGAGACCCCGCTATAAGTT	55.0	50.0	58.1	57.9	1541-1647 bp	1.0	91.0
accD-psaI_p4	TACTACAGGTGGGGTGACAG	TCGAGACCCCGCTATAAGTT	55.0	50.0	58.1	57.9	1540-1646 bp	1.0	91.0
accD-psaI_p5	TACTACAGGTGGGGTGACAG	TTCGAGACCCCGCTATAAGT	55.0	50.0	58.1	57.9	1541-1647 bp	1.0	91.0