Pi
0.042
MarkerSeek score
77.90
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | TTTTCCGGCGATATGAACGT | TAGCGTCTACCAATTTCGCC | 45.0 | 50.0 | 58.0 | 58.1 | 1345-1502 bp | 0.976744 | 90.4 |
| trnT-UGU-trnL-UAA_p2 | TTTTCCGGCGATATGAACGT | AAATGGGCCTTTTTGGGGAT | 45.0 | 45.0 | 58.0 | 57.9 | 1929-2085 bp | 0.790698 | 83.1 |
| trnT-UGU-trnL-UAA_p3 | TTTTCCGGCGATATGAACGT | ACCAATTTCGCCATATCCCC | 45.0 | 50.0 | 58.0 | 58.0 | 1337-1494 bp | 0.325581 | 61.9 |
| trnT-UGU-trnL-UAA_p4 | TGCTTTTCCGGCGATATGAA | ACCAATTTCGCCATATCCCC | 45.0 | 50.0 | 58.0 | 58.0 | 1340-1497 bp | 0.325581 | 61.8 |
| trnT-UGU-trnL-UAA_p5 | TTGCTTTTCCGGCGATATGA | ACCAATTTCGCCATATCCCC | 45.0 | 50.0 | 58.0 | 58.0 | 1341-1498 bp | 0.325581 | 61.8 |