Hotspot

trnE-UUC-trnT-GGU

LSC · 30961-31507 · 547 bp

Back to Elatostema

Pi 0.0434

MarkerSeek score 78.70

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 547
variable_sites: 129
pi: 0.0434
unique_haplotype_count: 39
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AGAGATGTCCTGAACCGCTA	ACGCCTTACCATGACGTTAC	50.0	50.0	57.9	58.0	342-629 bp	1.0	90.8
trnE-UUC-trnT-GGU_p2	AGATGTCCTGAACCGCTAGA	ACGCCTTACCATGACGTTAC	50.0	50.0	57.9	58.0	340-627 bp	1.0	90.8
trnE-UUC-trnT-GGU_p3	GATGTCCTGAACCGCTAGAC	ACGCCTTACCATGACGTTAC	55.0	50.0	57.8	58.0	339-626 bp	1.0	90.3
trnE-UUC-trnT-GGU_p4	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	355-642 bp	0.953488	88.9
trnE-UUC-trnT-GGU_p5	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	353-640 bp	0.953488	88.9