Hotspot

petN-psbM

LSC · 28893-29640 · 748 bp

Back to Elatostema

Pi 0.0437

MarkerSeek score 80.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 748
variable_sites: 167
pi: 0.0437
unique_haplotype_count: 40
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GGCAAATCGGGGATGGTAAT	AATCATCAAACCGCCCTACC	50.0	50.0	58.0	57.9	1702-1795 bp	0.186047	54.9
petN-psbM_p2	GGCAAATCGGGGATGGTAAT	TATAAACTAGTGCTCGCGCC	50.0	50.0	58.0	57.9	1751-1844 bp	0.186047	54.3
petN-psbM_p3	ATATGGCAAATCGGGGATGG	AATCATCAAACCGCCCTACC	50.0	50.0	57.8	57.9	1786-1799 bp	0.162791	51.8
petN-psbM_p4	GGCAAATCGGGGATGGTAAT	GCCCTACCGTTGCTTCTAAA	50.0	50.0	58.0	57.9	1772-1783 bp	0.139535	46.2
petN-psbM_p5	GGCAAATCGGGGATGGTAAT	TCAAATCATCAAACCGCCCT	50.0	45.0	58.0	57.8	1787-1798 bp	0.139535	45.3