Hotspot

rpoB-trnC-GCA

LSC · 26741-27792 · 1052 bp

Back to Elatostema

Pi 0.0388

MarkerSeek score 77.30

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1052
variable_sites: 216
pi: 0.0388
unique_haplotype_count: 40
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GATACCGGGGCTTTGCAATA	ATTACCATCCCCGATTTGCC	50.0	50.0	58.0	58.0	2041-2116 bp	0.186047	54.2
rpoB-trnC-GCA_p2	AGAGGTTCCCAGGGAATTCA	ATTACCATCCCCGATTTGCC	50.0	50.0	57.9	58.0	1984-2059 bp	0.186047	54.2
rpoB-trnC-GCA_p3	CCGGGGCTTTGCAATATTTG	ATTACCATCCCCGATTTGCC	50.0	50.0	58.1	58.0	2037-2112 bp	0.186047	54.0
rpoB-trnC-GCA_p4	ACACGAACCCATATCCTTGC	ATTACCATCCCCGATTTGCC	50.0	50.0	58.0	58.0	2160-2235 bp	0.186047	53.8
rpoB-trnC-GCA_p5	TCCTACTCACACGAACCCAT	ATTACCATCCCCGATTTGCC	50.0	50.0	58.1	58.0	2168-2243 bp	0.186047	53.6