Hotspot

trnS-GCU-trnG-UCC

LSC · 8491-9146 · 656 bp

Back to Elatostema

Pi 0.0428
MarkerSeek score 81.10
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
656
variable_sites
152
pi
0.0428
unique_haplotype_count
40
primer_available
yes
alignment_reliability
0.98
markerseek_score
81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 408-759 bp 0.953488 80.2
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 407-758 bp 0.953488 80.2
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA ACGAATCGCACTTTTACCAC 50.0 45.0 58.0 56.8 408-759 bp 0.953488 80.1
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC ACGAATCGCACTTTTACCAC 50.0 45.0 58.0 56.8 407-758 bp 0.953488 80.1
trnS-GCU-trnG-UCC_p5 CAATCCGACGCTTTAGTCCA AACGAATCGCACTTTTACCAC 50.0 42.9 58.0 57.5 409-760 bp 0.953488 77.9