Hotspot

rps16-trnQ-UUG

LSC · 6268-7032 · 765 bp

Back to Elatostema

Pi 0.0652
MarkerSeek score 82.70
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
765
variable_sites
250
pi
0.0652
unique_haplotype_count
40
primer_available
yes
alignment_reliability
0.97
markerseek_score
82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 548-905 bp 0.976744 89.3
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 552-909 bp 0.976744 84.6
rps16-trnQ-UUG_p3 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 540-897 bp 0.976744 84.2
rps16-trnQ-UUG_p4 AGTCGCACGTTGCTTTCTAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.9 58.1 547-904 bp 0.976744 83.6
rps16-trnQ-UUG_p5 GCACGTTGCTTTCTACCACA GAGGTTCGAATCCTTCCGTC 50.0 55.0 59.1 58.1 543-900 bp 0.976744 82.0