Hotspot

trnK-UUU-rps16

LSC · 4282-5056 · 775 bp

Back to Elatostema

Pi 0.0543
MarkerSeek score 81.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
775
variable_sites
208
pi
0.0543
unique_haplotype_count
40
primer_available
yes
alignment_reliability
0.97
markerseek_score
81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCGCGGTCTTACAAACTC AAAAGGGTATGTTGCTGCCA 50.0 45.0 57.9 58.0 1545-1676 bp 0.906977 87.7
trnK-UUU-rps16_p2 GTCGCGGTCTTACAAACTCT AAAAGGGTATGTTGCTGCCA 50.0 45.0 57.9 58.0 1544-1675 bp 0.906977 87.7
trnK-UUU-rps16_p3 GTCGCGGTCTTACAAACTCT TTGAAAAAGGTGCTCAGCCT 50.0 45.0 57.9 57.9 873-1014 bp 0.906977 87.2
trnK-UUU-rps16_p4 AGTCGCGGTCTTACAAACTC ATTTCAAAGAAGGCGGGTGT 50.0 45.0 57.9 58.0 852-977 bp 0.27907 59.8
trnK-UUU-rps16_p5 GTCGCGGTCTTACAAACTCT ATTTCAAAGAAGGCGGGTGT 50.0 45.0 57.9 58.0 851-976 bp 0.27907 59.8