Hotspot

rps16

LSC · 84426-85240 · 815 bp

Back to Elaphoglossum

Pi 0.0275

MarkerSeek score 65.60

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 815
variable_sites: 33
pi: 0.0275
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AAGTAGGGCAGCAATAGCAA	AGAAATTCATTCTCGGTTTTTAGA	45.0	29.2	57.2	54.8	873-976 bp	1.0	43.8
rps16_p2	AGTAGGGCAGCAATAGCAAA	AGAAATTCATTCTCGGTTTTTAGA	45.0	29.2	57.2	54.8	872-975 bp	1.0	43.8
rps16_p3	AAGTAGGGCAGCAATAGCAAA	AGAAATTCATTCTCGGTTTTTAGA	42.9	29.2	57.9	54.8	873-976 bp	1.0	43.8
rps16_p4	AAGTAGGGCAGCAATAGCAA	GAAATTCATTCTCGGTTTTTAGA	45.0	30.4	57.2	53.4	872-975 bp	1.0	43.8
rps16_p5	AGTAGGGCAGCAATAGCAAA	GAAATTCATTCTCGGTTTTTAGA	45.0	30.4	57.2	53.4	871-974 bp	1.0	43.8