Hotspot

trnW-CCA-trnP-UGG

LSC · 146161-146334 · 174 bp

Back to Elaphoglossum

Pi 0.0351
MarkerSeek score 67.20
Primer available yes
Label trnW-CCA-trnP-UGG

Feature Metadata

label_name
trnW-CCA-trnP-UGG
feature_type
igs
region
LSC
length_bp
174
variable_sites
9
pi
0.0351
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW-CCA-trnP-UGG_p1 ACCTACGACATCGGGTTTTG GGTAGCGTGTTTGTTTTGGG 50.0 50.0 57.9 57.9 285-292 bp 1.0 81.6
trnW-CCA-trnP-UGG_p2 ACCTACGACATCGGGTTTTG CGGTAGCGTGTTTGTTTTGG 50.0 50.0 57.9 58.3 286-293 bp 1.0 80.6
trnW-CCA-trnP-UGG_p3 GAAATAAACACGCCCTGCAG GGTAGCGTGTTTGTTTTGGG 50.0 50.0 57.7 57.9 312-319 bp 1.0 79.9
trnW-CCA-trnP-UGG_p4 CTCGGAAATAAACACGCCCT GGTAGCGTGTTTGTTTTGGG 50.0 50.0 58.3 57.9 316-323 bp 1.0 79.8
trnW-CCA-trnP-UGG_p5 TCCTCGGAAATAAACACGCC GGTAGCGTGTTTGTTTTGGG 50.0 50.0 58.3 57.9 318-325 bp 1.0 79.8