Hotspot

psbD-trnT-GGU

LSC · 117120-118467 · 1348 bp

Back to Elaphoglossum

Pi 0.0195
MarkerSeek score 65.00
Primer available yes
Label psbD-trnT-GGU

Feature Metadata

label_name
psbD-trnT-GGU
feature_type
igs
region
LSC
length_bp
1348
variable_sites
39
pi
0.0195
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbD-trnT-GGU_p1 CCACGAAGACGAAACGATCT CGTAAGTCGTCGGTTCAAGT 50.0 50.0 58.0 58.0 1475-1490 bp 1.0 83.1
psbD-trnT-GGU_p2 CCACGAAGACGAAACGATCT TCTGAAGCCCCCTTAACTCA 50.0 50.0 58.0 58.0 1520-1535 bp 1.0 83.0
psbD-trnT-GGU_p3 CGAAACGATCTCTCCTGAGC CGTAAGTCGTCGGTTCAAGT 55.0 50.0 58.2 58.0 1466-1481 bp 1.0 81.9
psbD-trnT-GGU_p4 CGAAACGATCTCTCCTGAGC TCTGAAGCCCCCTTAACTCA 55.0 50.0 58.2 58.0 1511-1526 bp 1.0 81.8
psbD-trnT-GGU_p5 GAAACGATCTCTCCTGAGCC TCTGAAGCCCCCTTAACTCA 55.0 50.0 57.8 58.0 1510-1525 bp 1.0 81.4