Hotspot

atpH-atpI

LSC · 93759-94545 · 787 bp

Back to Elaphoglossum

Pi 0.0226

MarkerSeek score 66.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 787
variable_sites: 26
pi: 0.0226
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GCAGCAATAACAGAAGCAGC	CTGCGGCTTATATTGGGGAA	50.0	50.0	58.1	57.7	842-857 bp	1.0	81.4
atpH-atpI_p2	CGAGACCTACAGCTAACCCA	CTGCGGCTTATATTGGGGAA	55.0	50.0	58.5	57.7	862-877 bp	1.0	77.8
atpH-atpI_p3	CCAGCAGCAATAACAGAAGC	CTGCGGCTTATATTGGGGAA	50.0	50.0	57.4	57.7	845-860 bp	1.0	77.7
atpH-atpI_p4	AACCCAGCAGCAATAACAGA	CTGCGGCTTATATTGGGGAA	45.0	50.0	57.4	57.7	848-863 bp	1.0	77.5
atpH-atpI_p5	ACCCAGCAGCAATAACAGAA	CTGCGGCTTATATTGGGGAA	45.0	50.0	57.4	57.7	847-862 bp	1.0	77.5