Hotspot

ndhB-matK

LSC · 81913-82147 · 235 bp

Back to Elaphoglossum

Pi 0.0298

MarkerSeek score 64.30

Primer available yes

Label ndhB-matK

Feature Metadata

label_name: ndhB-matK
feature_type: igs
region: LSC
length_bp: 235
variable_sites: 10
pi: 0.0298
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-matK_p1	TGACAAATTACTAAGTAAAACGA	ACTGCTCTGTTTCATTAACA	26.1	35.0	52.1	52.7	291-303 bp	1.0	45.9
ndhB-matK_p2	TGACAAATTACTAAGTAAAACGA	AACTGCTCTGTTTCATTAACA	26.1	33.3	52.1	53.7	292-304 bp	1.0	45.9
ndhB-matK_p3	TGACAAATTACTAAGTAAAACGAA	ACTGCTCTGTTTCATTAACA	25.0	35.0	53.0	52.7	291-303 bp	1.0	45.9
ndhB-matK_p4	TTGACAAATTACTAAGTAAAACGA	ACTGCTCTGTTTCATTAACA	25.0	35.0	53.0	52.7	292-304 bp	1.0	45.9
ndhB-matK_p5	TGACAAATTACTAAGTAAAACGA	AAACTGCTCTGTTTCATTAACA	26.1	31.8	52.1	54.5	293-305 bp	1.0	45.8