Hotspot

ycf1

SSC · 126714-132299 · 5586 bp

Back to Elaeocarpus

Pi 0.0143

MarkerSeek score 45.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5586
variable_sites: 209
pi: 0.0143
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTATCGTTGGGGTTGGCAA	TCGTCGGAATCGTCCATTTC	45.0	50.0	58.0	58.1	733-742 bp	1.0	84.6
ycf1_p2	TTTTATCGTTGGGGTTGGCA	TCGTCGGAATCGTCCATTTC	45.0	50.0	58.0	58.1	734-743 bp	1.0	84.6
ycf1_p3	TTTATCGTTGGGGTTGGCAA	GAAGGGGACTAAACGGGAAC	45.0	55.0	58.0	57.9	961-973 bp	1.0	84.6
ycf1_p4	TTTTATCGTTGGGGTTGGCA	GAAGGGGACTAAACGGGAAC	45.0	55.0	58.0	57.9	962-974 bp	1.0	84.6
ycf1_p5	GGAGTTCCCAAGAAACCGTT	TCGTCGGAATCGTCCATTTC	50.0	50.0	58.0	58.1	1064-1073 bp	1.0	83.5