Hotspot

ndhA

SSC · 122773-125059 · 2287 bp

Back to Elaeocarpus

Pi 0.0112

MarkerSeek score 52.30

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2287
variable_sites: 62
pi: 0.0112
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGAGACAAGTCATGAATATAGT	TGGGAGAAGTTGATCGTTGA	31.8	45.0	52.1	56.5	2298-2353 bp	1.0	44.8
ndhA_p2	TTGAGACAAGTCATGAATATAGT	TGGGAGAAGTTGATCGTTGA	30.4	45.0	53.0	56.5	2299-2354 bp	1.0	44.8
ndhA_p3	TGAGACAAGTCATGAATATAGT	TGGGAGAAGTTGATCGTTGAA	31.8	42.9	52.1	57.2	2298-2353 bp	1.0	44.8
ndhA_p4	TTGAGACAAGTCATGAATATAGT	TGGGAGAAGTTGATCGTTGAA	30.4	42.9	53.0	57.2	2299-2354 bp	1.0	44.8
ndhA_p5	TGAGACAAGTCATGAATATAGT	ATGGGAGAAGTTGATCGTTGA	31.8	42.9	52.1	57.0	2299-2354 bp	1.0	44.8