Hotspot

ndhF-trnL-UAG

SSC · 115848-117222 · 1375 bp

Back to Elaeocarpus

Pi 0.0143
MarkerSeek score 60.70
Primer available yes
Label ndhF-trnL-UAG

Feature Metadata

label_name
ndhF-trnL-UAG
feature_type
igs
region
SSC
length_bp
1375
variable_sites
52
pi
0.0143
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.97
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-trnL-UAG_p1 AGCCCCGCTCCTATTAACAT AGCGTGTCTACCGATTTCAC 50.0 50.0 58.6 58.0 1208-1567 bp 1.0 82.2
ndhF-trnL-UAG_p2 AGCCCCGCTCCTATTAACAT CAGCGTGTCTACCGATTTCA 50.0 50.0 58.6 58.0 1209-1568 bp 1.0 82.2
ndhF-trnL-UAG_p3 TTTTGTTGCCGTCGGAAAAA AGCGTGTCTACCGATTTCAC 40.0 50.0 57.4 58.0 1233-1592 bp 1.0 81.5
ndhF-trnL-UAG_p4 TTTTTGTTGCCGTCGGAAAA AGCGTGTCTACCGATTTCAC 40.0 50.0 57.4 58.0 1234-1593 bp 1.0 81.5
ndhF-trnL-UAG_p5 TTTTGTTGCCGTCGGAAAAA CAGCGTGTCTACCGATTTCA 40.0 50.0 57.4 58.0 1234-1593 bp 1.0 81.5