Hotspot

psaJ-rpl33

LSC · 70614-70917 · 304 bp

Back to Elaeocarpus

Pi 0.0184

MarkerSeek score 62.70

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 304
variable_sites: 16
pi: 0.0184
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTATGGTTCGGGGCTTTAGC	TCGTTTCGAACACAACTGGT	50.0	45.0	58.0	58.0	443-456 bp	1.0	84.9
psaJ-rpl33_p2	CAGATGCGTTGACATTCCCT	TCGTTTCGAACACAACTGGT	50.0	45.0	58.3	58.0	391-404 bp	1.0	83.9
psaJ-rpl33_p3	TCCAGATGCGTTGACATTCC	TCGTTTCGAACACAACTGGT	50.0	45.0	58.3	58.0	393-406 bp	1.0	83.8
psaJ-rpl33_p4	TTATGGTTCGGGGCTTTAGC	GGACATCTTTACCCTTGGCC	50.0	55.0	58.0	58.2	400-413 bp	1.0	83.7
psaJ-rpl33_p5	TGGCACCGGTACTAAGTACT	TCGTTTCGAACACAACTGGT	50.0	45.0	57.8	58.0	463-476 bp	1.0	83.3