Hotspot

petA-psbJ

LSC · 66060-67033 · 974 bp

Back to Elaeocarpus

Pi 0.00794

MarkerSeek score 54.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 974
variable_sites: 24
pi: 0.00794
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAGAAGGTTCAATTGTCCGA	TGGTTCATATTCTGGATTGGGT	42.9	40.9	57.2	57.1	1012-1042 bp	1.0	47.1
petA-psbJ_p2	TGAGAAGGTTCAATTGTCCGA	ATTCTGGATTGGGTTCATCC	42.9	45.0	57.2	55.1	1004-1034 bp	1.0	46.6
petA-psbJ_p3	TGAGAAGGTTCAATTGTCCGAA	TGGTTCATATTCTGGATTGGGT	40.9	40.9	57.9	57.1	1012-1042 bp	1.0	44.6
petA-psbJ_p4	TTGAGAAGGTTCAATTGTCCGA	TGGTTCATATTCTGGATTGGGT	40.9	40.9	57.9	57.1	1013-1043 bp	1.0	44.6
petA-psbJ_p5	TGAGAAGGTTCAATTGTCCGA	TGGTTCATATTCTGGATTGGGTT	42.9	39.1	57.2	57.8	1012-1042 bp	1.0	44.5