Hotspot

ycf4-cemA

LSC · 63550-64163 · 614 bp

Back to Elaeocarpus

Pi 0.0117

MarkerSeek score 61.10

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 614
variable_sites: 21
pi: 0.0117
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGCGCGTACCAATTGAAGTA	GAGAGAGAGATCCACCAGGG	45.0	60.0	57.9	58.3	681-717 bp	1.0	83.4
ycf4-cemA_p2	CTTGCGCGTACCAATTGAAG	GAGAGAGAGATCCACCAGGG	50.0	60.0	58.1	58.3	683-719 bp	1.0	83.1
ycf4-cemA_p3	TGCGCGTACCAATTGAAGTA	TGAGAGAGAGATCCACCAGG	45.0	55.0	57.9	57.5	682-718 bp	1.0	82.2
ycf4-cemA_p4	CTTGCGCGTACCAATTGAAG	TGAGAGAGAGATCCACCAGG	50.0	55.0	58.1	57.5	684-720 bp	1.0	81.9
ycf4-cemA_p5	TTTCTTGCGCGTACCAATTG	GAGAGAGAGATCCACCAGGG	45.0	60.0	57.7	58.3	686-722 bp	1.0	81.5