Hotspot

psbZ-trnG-GCC

LSC · 38291-38826 · 536 bp

Back to Elaeocarpus

Pi 0.0323

MarkerSeek score 75.20

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 536
variable_sites: 47
pi: 0.0323
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CGATGGTTGGTCGAGTAACA	AATCGAACCCGCATCTTCTC	50.0	50.0	57.6	58.1	677-786 bp	1.0	85.9
psbZ-trnG-GCC_p2	CGATGGTTGGTCGAGTAACA	GAATCGAACCCGCATCTTCT	50.0	50.0	57.6	58.1	678-787 bp	1.0	85.9
psbZ-trnG-GCC_p3	CCGATGGTTGGTCGAGTAAC	AATCGAACCCGCATCTTCTC	55.0	50.0	58.4	58.1	678-787 bp	1.0	85.9
psbZ-trnG-GCC_p4	CCGATGGTTGGTCGAGTAAC	GAATCGAACCCGCATCTTCT	55.0	50.0	58.4	58.1	679-788 bp	1.0	85.9
psbZ-trnG-GCC_p5	TTTGCTTCTCCCGATGGTTG	AATCGAACCCGCATCTTCTC	50.0	50.0	58.5	58.1	688-797 bp	1.0	85.0