Hotspot

trnT-GGU-psbD

LSC · 33491-34990 · 1500 bp

Back to Elaeocarpus

Pi 0.00691

MarkerSeek score 54.10

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1500
variable_sites: 33
pi: 0.00691
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGGAAAGAGCAATAGACCGG	55.0	55.0	58.1	58.0	1654-1678 bp	1.0	83.0
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GGGAAAGAGCAATAGACCGG	50.0	55.0	58.1	58.0	1657-1681 bp	1.0	82.8
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1626-1650 bp	1.0	81.4
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	GACCGGACCAACCTACAAAA	55.0	50.0	58.1	57.7	1640-1664 bp	1.0	81.3
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	GGGAAAGAGCAATAGACCGG	50.0	55.0	58.3	58.0	1631-1655 bp	1.0	81.3