Hotspot

psbK-psbI

LSC · 7419-7864 · 446 bp

Back to Elaeocarpus

Pi 0.0118

MarkerSeek score 55.70

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 446
variable_sites: 15
pi: 0.0118
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CTTTTGTTTGGCAAGCTGCT	CCGAAGATGAAGAGAGAAACA	45.0	42.9	58.1	55.4	527-574 bp	1.0	56.7
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	CCGAAGATGAAGAGAGAAACA	50.0	42.9	57.9	55.4	522-569 bp	1.0	56.7
psbK-psbI_p3	GTTTGGCAAGCTGCTGTAAG	CCGAAGATGAAGAGAGAAACAA	50.0	40.9	57.9	56.1	522-569 bp	1.0	54.8
psbK-psbI_p4	CTTTTGTTTGGCAAGCTGCT	CCGAAGATGAAGAGAGAAACAA	45.0	40.9	58.1	56.1	527-574 bp	1.0	54.7
psbK-psbI_p5	TGGCAAGCTGCTGTAAGTTT	CCGAAGATGAAGAGAGAAACA	45.0	42.9	58.3	55.4	519-566 bp	1.0	54.7