primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	20	20	55.0	55.0	58.1	57.9	0.0	13.4	0.194818	102	467	423-451	1.000000	85.7
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	102	467	426-454	1.000000	85.6
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.199180	102	467	437-465	1.000000	85.5
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	102	467	436-464	1.000000	85.5
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	102	467	438-466	1.000000	85.4
trnK-UUU_p1	trnK-UUU	1	GGACTCCCAAGCACACAAAT	TGTTTTGATTGTGTCGTGCA	20	20	50.0	40.0	58.4	56.8	0.0	0.0	1.592751	1811	4438	2934-2977	1.000000	70.3
trnK-UUU_p2	trnK-UUU	2	GGACTCCCAAGCACACAAAT	TGTTTTGATTGTGTCGTGCAA	20	21	50.0	38.1	58.4	57.5	0.0	0.0	1.873222	1811	4438	2934-2977	1.000000	68.1
trnK-UUU_p3	trnK-UUU	3	GGACTCCCAAGCACACAAAT	ATGTTTTGATTGTGTCGTGCA	20	21	50.0	38.1	58.4	57.3	0.0	0.0	2.085979	1811	4438	2935-2978	1.000000	66.4
trnK-UUU_p4	trnK-UUU	4	ACTCCCAAGCACACAAATTCT	TGTTTTGATTGTGTCGTGCA	21	20	42.9	40.0	58.1	56.8	0.0	0.0	2.264126	1811	4438	2932-2975	1.000000	65.0
trnK-UUU_p5	trnK-UUU	5	GGACTCCCAAGCACACAAA	TGTTTTGATTGTGTCGTGCA	19	20	52.6	40.0	57.9	56.8	0.0	0.0	2.323421	1811	4438	2934-2977	1.000000	64.5
matK-trnK-UUU_p1	matK-trnK-UUU	1	GGAAGTCATGTTGCCGAGAT	AACGGTAGAGTACTCGGCTT	20	20	50.0	50.0	58.0	58.2	0.0	17.1	0.198258	3625	4401	833-872	1.000000	82.9
matK-trnK-UUU_p2	matK-trnK-UUU	2	GGAAGTCATGTTGCCGAGAT	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	58.0	58.2	0.0	17.1	0.198258	3625	4401	832-871	1.000000	82.9
matK-trnK-UUU_p3	matK-trnK-UUU	3	GGAAGTCATGTTGCCGAGAT	TTGGGTTGCTAACTCAACGG	20	20	50.0	50.0	58.0	58.4	0.0	9.4	0.429815	3625	4401	848-887	1.000000	81.0
matK-trnK-UUU_p4	matK-trnK-UUU	4	GGAAGTCATGTTGCCGAGAT	GGGTTGCTAACTCAACGGTA	20	20	50.0	50.0	58.0	57.5	0.0	0.0	0.490595	3625	4401	846-885	1.000000	80.5
matK-trnK-UUU_p5	matK-trnK-UUU	5	TAGGAAGTCATGTTGCCGAG	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	57.3	58.2	0.0	17.1	0.841381	3625	4401	834-873	1.000000	77.8
psbK-psbI_p1	psbK-psbI	1	CTTTTGTTTGGCAAGCTGCT	CCGAAGATGAAGAGAGAAACA	20	21	45.0	42.9	58.1	55.4	15.2	0.0	3.688483	7419	7864	527-574	1.000000	56.7
psbK-psbI_p2	psbK-psbI	2	GTTTGGCAAGCTGCTGTAAG	CCGAAGATGAAGAGAGAAACA	20	21	50.0	42.9	57.9	55.4	1.4	0.0	3.688608	7419	7864	522-569	1.000000	56.7
psbK-psbI_p3	psbK-psbI	3	GTTTGGCAAGCTGCTGTAAG	CCGAAGATGAAGAGAGAAACAA	20	22	50.0	40.9	57.9	56.1	1.4	0.0	3.930843	7419	7864	522-569	1.000000	54.8
psbK-psbI_p4	psbK-psbI	4	CTTTTGTTTGGCAAGCTGCT	CCGAAGATGAAGAGAGAAACAA	20	22	45.0	40.9	58.1	56.1	15.2	0.0	3.930717	7419	7864	527-574	1.000000	54.7
psbK-psbI_p5	psbK-psbI	5	TGGCAAGCTGCTGTAAGTTT	CCGAAGATGAAGAGAGAAACA	20	21	45.0	42.9	58.3	55.4	1.4	0.0	3.941318	7419	7864	519-566	1.000000	54.7
atpF-atpH_p1	atpF-atpH	1	GGGATCATGCACCTTTTCCT	ATTTCTGCCGCTTCCGTTAT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.188360	13522	14233	1047-1175	1.000000	84.9
atpF-atpH_p2	atpF-atpH	2	GGGATCATGCACCTTTTCCT	CGACTGGATGAATCTTGGCA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.178393	13522	14233	1155-1283	1.000000	84.8
atpF-atpH_p3	atpF-atpH	3	CATGCTGCTTCTGGACTTCA	ATTTCTGCCGCTTCCGTTAT	20	20	50.0	45.0	58.2	58.0	0.0	0.0	0.221029	13522	14233	1206-1334	1.000000	84.3
atpF-atpH_p4	atpF-atpH	4	CATGCTGCTTCTGGACTTCA	CGACTGGATGAATCTTGGCA	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.211062	13522	14233	1314-1442	1.000000	84.2
atpF-atpH_p5	atpF-atpH	5	GGGATCATGCACCTTTTCCT	AAGCCGTAGAAGGTATTGCG	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.214040	13522	14233	1086-1086	0.111111	42.7
petN-psbM_p1	petN-psbM	1	TAGTAAATCTCGCCTGGGCT	TGCTACTGCACTGTTCATTT	20	20	50.0	40.0	58.2	55.6	10.0	0.0	2.633070	30242	30978	843-899	1.000000	63.9
petN-psbM_p2	petN-psbM	2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTT	20	20	50.0	40.0	57.6	55.6	0.0	0.0	2.773455	30242	30978	782-838	1.000000	62.8
petN-psbM_p3	petN-psbM	3	TAGTAAATCTCGCCTGGGCT	TGCTACTGCACTGTTCATTTT	20	21	50.0	38.1	58.2	56.4	10.0	0.0	2.844881	30242	30978	843-899	1.000000	62.2
petN-psbM_p4	petN-psbM	4	CCTGGGCTGCTTTAATGGTA	TGCTACTGCACTGTTCATTT	20	20	50.0	40.0	57.6	55.6	0.0	0.0	2.857965	30242	30978	831-887	1.000000	62.1
petN-psbM_p5	petN-psbM	5	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTTT	20	21	50.0	38.1	57.6	56.4	0.0	0.0	2.985266	30242	30978	782-838	1.000000	61.1
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	GCATCGACCCAGGAAGAATT	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.050093	32746	33418	830-877	1.000000	86.3
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	GATTAGAGTCGCCGTGTTGT	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.013801	32746	33418	1209-1258	1.000000	85.6
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	GATTAGAGTCGCCGTGTTGT	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.071898	32746	33418	1188-1237	1.000000	85.3
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	GATTAGAGTCGCCGTGTTGT	TGGCGTTACTCTACCACTGA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.094052	32746	33418	1185-1234	1.000000	85.1
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	TTGGATTTTGGTCTGTCGGG	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.101684	32746	33418	1379-1418	0.777778	75.1
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	GGGAAAGAGCAATAGACCGG	20	20	55.0	55.0	58.1	58.0	7.6	0.0	0.109140	33491	34990	1654-1678	1.000000	83.0
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	GGGAAAGAGCAATAGACCGG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.131295	33491	34990	1657-1681	1.000000	82.8
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	20	20	55.0	45.0	58.1	58.2	7.6	0.0	0.310137	33491	34990	1626-1650	1.000000	81.4
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GTGGTAGAGTAACGCCATGG	GACCGGACCAACCTACAAAA	20	20	55.0	50.0	58.1	57.7	7.6	9.5	0.323334	33491	34990	1640-1664	1.000000	81.3
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GGCGTAAGTCATCGGTTCAA	GGGAAAGAGCAATAGACCGG	20	20	50.0	55.0	58.3	58.0	0.0	0.0	0.327879	33491	34990	1631-1655	1.000000	81.3
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	CGATGGTTGGTCGAGTAACA	AATCGAACCCGCATCTTCTC	20	20	50.0	50.0	57.6	58.1	12.2	0.0	0.416002	38291	38826	677-786	1.000000	85.9
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	CGATGGTTGGTCGAGTAACA	GAATCGAACCCGCATCTTCT	20	20	50.0	50.0	57.6	58.1	12.2	0.0	0.416002	38291	38826	678-787	1.000000	85.9
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	CCGATGGTTGGTCGAGTAAC	AATCGAACCCGCATCTTCTC	20	20	55.0	50.0	58.4	58.1	3.7	0.0	0.421687	38291	38826	678-787	1.000000	85.9
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	CCGATGGTTGGTCGAGTAAC	GAATCGAACCCGCATCTTCT	20	20	55.0	50.0	58.4	58.1	3.7	0.0	0.421687	38291	38826	679-788	1.000000	85.9
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	TTTGCTTCTCCCGATGGTTG	AATCGAACCCGCATCTTCTC	20	20	50.0	50.0	58.5	58.1	0.0	0.0	0.525050	38291	38826	688-797	1.000000	85.0
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	AATAGAGAAAAGCCAGCCGG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.048746	48989	50057	1148-1176	1.000000	84.1
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	AATAGAGAAAAGCCAGCCGG	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.113555	48989	50057	1156-1184	1.000000	83.6
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	48989	50057	1091-1119	1.000000	83.6
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	48989	50057	1099-1127	1.000000	83.1
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	48989	50057	1123-1151	1.000000	82.6
ycf4-cemA_p1	ycf4-cemA	1	TGCGCGTACCAATTGAAGTA	GAGAGAGAGATCCACCAGGG	20	20	45.0	60.0	57.9	58.3	8.4	0.0	0.378315	63550	64163	681-717	1.000000	83.4
ycf4-cemA_p2	ycf4-cemA	2	CTTGCGCGTACCAATTGAAG	GAGAGAGAGATCCACCAGGG	20	20	50.0	60.0	58.1	58.3	8.4	0.0	0.414391	63550	64163	683-719	1.000000	83.1
ycf4-cemA_p3	ycf4-cemA	3	TGCGCGTACCAATTGAAGTA	TGAGAGAGAGATCCACCAGG	20	20	45.0	55.0	57.9	57.5	8.4	0.0	0.532593	63550	64163	682-718	1.000000	82.2
ycf4-cemA_p4	ycf4-cemA	4	CTTGCGCGTACCAATTGAAG	TGAGAGAGAGATCCACCAGG	20	20	50.0	55.0	58.1	57.5	8.4	0.0	0.568669	63550	64163	684-720	1.000000	81.9
ycf4-cemA_p5	ycf4-cemA	5	TTTCTTGCGCGTACCAATTG	GAGAGAGAGATCCACCAGGG	20	20	45.0	60.0	57.7	58.3	8.4	0.0	0.620529	63550	64163	686-722	1.000000	81.5
petA-psbJ_p1	petA-psbJ	1	TGAGAAGGTTCAATTGTCCGA	TGGTTCATATTCTGGATTGGGT	21	22	42.9	40.9	57.2	57.1	5.1	0.0	4.641702	66060	67033	1012-1042	1.000000	47.1
petA-psbJ_p2	petA-psbJ	2	TGAGAAGGTTCAATTGTCCGA	ATTCTGGATTGGGTTCATCC	21	20	42.9	45.0	57.2	55.1	5.1	0.0	4.709356	66060	67033	1004-1034	1.000000	46.6
petA-psbJ_p3	petA-psbJ	3	TGAGAAGGTTCAATTGTCCGAA	TGGTTCATATTCTGGATTGGGT	22	22	40.9	40.9	57.9	57.1	5.1	0.0	4.959275	66060	67033	1012-1042	1.000000	44.6
petA-psbJ_p4	petA-psbJ	4	TTGAGAAGGTTCAATTGTCCGA	TGGTTCATATTCTGGATTGGGT	22	22	40.9	40.9	57.9	57.1	5.1	0.0	4.959275	66060	67033	1013-1043	1.000000	44.6
petA-psbJ_p5	petA-psbJ	5	TGAGAAGGTTCAATTGTCCGA	TGGTTCATATTCTGGATTGGGTT	21	23	42.9	39.1	57.2	57.8	5.1	0.0	4.967924	66060	67033	1012-1042	1.000000	44.5
psaJ-rpl33_p1	psaJ-rpl33	1	TTATGGTTCGGGGCTTTAGC	TCGTTTCGAACACAACTGGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.059939	70614	70917	443-456	1.000000	84.9
psaJ-rpl33_p2	psaJ-rpl33	2	CAGATGCGTTGACATTCCCT	TCGTTTCGAACACAACTGGT	20	20	50.0	45.0	58.3	58.0	0.0	0.0	0.270176	70614	70917	391-404	1.000000	83.9
psaJ-rpl33_p3	psaJ-rpl33	3	TCCAGATGCGTTGACATTCC	TCGTTTCGAACACAACTGGT	20	20	50.0	45.0	58.3	58.0	0.0	0.0	0.275099	70614	70917	393-406	1.000000	83.8
psaJ-rpl33_p4	psaJ-rpl33	4	TTATGGTTCGGGGCTTTAGC	GGACATCTTTACCCTTGGCC	20	20	50.0	55.0	58.0	58.2	0.0	0.0	0.283034	70614	70917	400-413	1.000000	83.7
psaJ-rpl33_p5	psaJ-rpl33	5	TGGCACCGGTACTAAGTACT	TCGTTTCGAACACAACTGGT	20	20	50.0	45.0	57.8	58.0	6.9	0.0	0.233590	70614	70917	463-476	1.000000	83.3
trnN-GUU-ndhF_p1	trnN-GUU-ndhF	1	GGCAGAATAGCAGAGCAAGT	AGTTTGTAACAAACGCAGCG	20	20	50.0	45.0	58.0	57.9	0.0	14.8	0.135163	112061	113873	2368-2854	1.000000	85.0
trnN-GUU-ndhF_p2	trnN-GUU-ndhF	2	GGCAGAATAGCAGAGCAAGT	TTGTTCGTTGGAGCCATAGG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.143480	112061	113873	2498-2984	1.000000	84.8
trnN-GUU-ndhF_p3	trnN-GUU-ndhF	3	GGCAGAATAGCAGAGCAAGT	TTCCACAGCAGGATTAACCG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.143480	112061	113873	2868-2926	0.555556	65.8
trnN-GUU-ndhF_p4	trnN-GUU-ndhF	4	GGCAGAATAGCAGAGCAAGT	ATAGCTTGTTCCACAGCAGG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.155331	112061	113873	2876-2934	0.555556	65.7
trnN-GUU-ndhF_p5	trnN-GUU-ndhF	5	GGCAGAATAGCAGAGCAAGT	ACAGCAGGATTAACCGCATT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.164301	112061	113873	2864-2922	0.555556	65.7
ndhF-trnL-UAG_p1	ndhF-trnL-UAG	1	AGCCCCGCTCCTATTAACAT	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.6	58.0	0.0	0.0	0.572574	115848	117222	1208-1567	1.000000	82.2
ndhF-trnL-UAG_p2	ndhF-trnL-UAG	2	AGCCCCGCTCCTATTAACAT	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.6	58.0	0.0	0.0	0.573945	115848	117222	1209-1568	1.000000	82.2
ndhF-trnL-UAG_p3	ndhF-trnL-UAG	3	TTTTGTTGCCGTCGGAAAAA	AGCGTGTCTACCGATTTCAC	20	20	40.0	50.0	57.4	58.0	17.5	0.0	0.657161	115848	117222	1233-1592	1.000000	81.5
ndhF-trnL-UAG_p4	ndhF-trnL-UAG	4	TTTTTGTTGCCGTCGGAAAA	AGCGTGTCTACCGATTTCAC	20	20	40.0	50.0	57.4	58.0	17.5	0.0	0.657161	115848	117222	1234-1593	1.000000	81.5
ndhF-trnL-UAG_p5	ndhF-trnL-UAG	5	TTTTGTTGCCGTCGGAAAAA	CAGCGTGTCTACCGATTTCA	20	20	40.0	50.0	57.4	58.0	17.5	0.0	0.658532	115848	117222	1234-1593	1.000000	81.5
ndhA_p1	ndhA	1	TGAGACAAGTCATGAATATAGT	TGGGAGAAGTTGATCGTTGA	22	20	31.8	45.0	52.1	56.5	0.0	0.0	9.421833	122773	125059	2298-2353	1.000000	44.8
ndhA_p2	ndhA	2	TTGAGACAAGTCATGAATATAGT	TGGGAGAAGTTGATCGTTGA	23	20	30.4	45.0	53.0	56.5	0.0	0.0	9.535924	122773	125059	2299-2354	1.000000	44.8
ndhA_p3	ndhA	3	TGAGACAAGTCATGAATATAGT	TGGGAGAAGTTGATCGTTGAA	22	21	31.8	42.9	52.1	57.2	0.0	0.0	9.671635	122773	125059	2298-2353	1.000000	44.8
ndhA_p4	ndhA	4	TTGAGACAAGTCATGAATATAGT	TGGGAGAAGTTGATCGTTGAA	23	21	30.4	42.9	53.0	57.2	0.0	0.0	9.785727	122773	125059	2299-2354	1.000000	44.8
ndhA_p5	ndhA	5	TGAGACAAGTCATGAATATAGT	ATGGGAGAAGTTGATCGTTGA	22	21	31.8	42.9	52.1	57.0	0.0	0.0	9.890299	122773	125059	2299-2354	1.000000	44.8
ycf1_p1	ycf1	1	TTTATCGTTGGGGTTGGCAA	TCGTCGGAATCGTCCATTTC	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.085929	130805	130924	733-742	1.000000	84.6
ycf1_p2	ycf1	2	TTTTATCGTTGGGGTTGGCA	TCGTCGGAATCGTCCATTTC	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.085929	130805	130924	734-743	1.000000	84.6
ycf1_p3	ycf1	3	TTTATCGTTGGGGTTGGCAA	GAAGGGGACTAAACGGGAAC	20	20	45.0	55.0	58.0	57.9	0.0	0.0	0.108208	130805	130924	961-973	1.000000	84.6
ycf1_p4	ycf1	4	TTTTATCGTTGGGGTTGGCA	GAAGGGGACTAAACGGGAAC	20	20	45.0	55.0	58.0	57.9	0.0	0.0	0.108208	130805	130924	962-974	1.000000	84.6
ycf1_p5	ycf1	5	GGAGTTCCCAAGAAACCGTT	TCGTCGGAATCGTCCATTTC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.105671	130805	130924	1064-1073	1.000000	83.5