Hotspot

ycf1

SSC · 120769-126345 · 5577 bp

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Pi 0.0117

MarkerSeek score 48.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5577
variable_sites: 258
pi: 0.0117
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCGTTATTCATTCCCATCCA	GGATCAATCCTGACATGAAT	40.0	40.0	54.1	52.7	163 bp	0.083333	3.3
ycf1_p2	TCGTTATTCATTCCCATCCAA	GGATCAATCCTGACATGAAT	38.1	40.0	55.0	52.7	163 bp	0.083333	3.3
ycf1_p3	TTCGTTATTCATTCCCATCCA	GGATCAATCCTGACATGAAT	38.1	40.0	55.0	52.7	164 bp	0.083333	3.3
ycf1_p4	TTCGTTATTCATTCCCATCCAA	GGATCAATCCTGACATGAAT	36.4	40.0	55.8	52.7	164 bp	0.083333	3.3
ycf1_p5	TTTCGTTATTCATTCCCATCCA	GGATCAATCCTGACATGAAT	36.4	40.0	55.8	52.7	165 bp	0.083333	3.3