Hotspot

rps19

LSC · 80828-81106 · 279 bp

Back to Elaeagnus

Pi 0.00831

MarkerSeek score 31.70

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: LSC
length_bp: 279
variable_sites: 10
pi: 0.00831
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	ATTTTTGGCTCGTGCAAACC	AACAGTCGGACAAGTAGGGA	45.0	50.0	58.1	58.0	1571-1606 bp	1.0	87.3
rps19_p2	ATTTTTGGCTCGTGCAAACC	ACAGTCGGACAAGTAGGGAA	45.0	50.0	58.1	58.0	1570-1605 bp	1.0	87.3
rps19_p3	CCCTCGTTGACTTCAGCTTT	AACAGTCGGACAAGTAGGGA	50.0	50.0	58.1	58.0	1223-1245 bp	1.0	87.1
rps19_p4	CCCTCGTTGACTTCAGCTTT	ACAGTCGGACAAGTAGGGAA	50.0	50.0	58.1	58.0	1222-1244 bp	1.0	87.1
rps19_p5	TTTTCACAGTAGTGCCCTCG	ACAGTCGGACAAGTAGGGAA	50.0	50.0	57.8	58.0	1240-1242 bp	0.166667	47.4