Hotspot

rpl2-trnH-GUG

IRa · 150282-150379 · 98 bp

Back to Elaeagnus

Pi 0.0567

MarkerSeek score 70.30

Primer available yes

Label rpl2-trnH-GUG

Feature Metadata

label_name: rpl2-trnH-GUG
feature_type: igs
region: IRa
length_bp: 98
variable_sites: 31
pi: 0.0567
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-trnH-GUG_p1	CTTCGGCGCCGTAGTAAATA	CGGATGTAGCCAAGTGGATC	50.0	55.0	57.9	58.1	187-205 bp	1.0	90.0
rpl2-trnH-GUG_p2	TTCGGCGCCGTAGTAAATAG	CGGATGTAGCCAAGTGGATC	50.0	55.0	57.9	58.1	186-204 bp	1.0	90.0
rpl2-trnH-GUG_p3	CTTCGGCGCCGTAGTAAATA	TGGATCAAGGCAGTGGATTG	50.0	50.0	57.9	57.9	173-191 bp	1.0	89.9
rpl2-trnH-GUG_p4	TTCGGCGCCGTAGTAAATAG	TGGATCAAGGCAGTGGATTG	50.0	50.0	57.9	57.9	172-190 bp	1.0	89.9
rpl2-trnH-GUG_p5	TTCGGCGCCGTAGTAAATAG	GGATCAAGGCAGTGGATTGT	50.0	50.0	57.9	57.9	171-189 bp	1.0	89.9